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Coronavirus Update:What patients and families need to know

The Children's National Research Institute

Research Projects

Pediatric Airway Epithelial Cell Model

Airway epithelial cells (AEC) are quite difficult to access in newborns and infants. To overcome this barrier our laboratory has adapted conditionally reprogrammed cell (CRC) technology to generate continuous primary cell cultures from nasal and bronchial AEC of young children.

CRC methods allow AEC to expand much faster and to survive multiple subcultures while preserving the original phenotype.

After multiple subcultures, the conditionally reprogrammed cells can differentiate into respiratory epithelium when grown in air-liquid interface and exhibit robust immune responses. Thus, our cell model system is a powerful translational approach to investigate the developmental immunobiology of the airway epithelium in humans and represents a new tool for scientists investigating the mechanisms behind viral-induced wheezing, asthma and other respiratory diseases that get their start in early childhood