IDDRC: Genomics and Proteomics Core
The Genomics, Proteomics, and Bioinformatics (GPB) Core of the Intellectual and Developmental Disabilities Research Center (IDDRC) facilitates clinical and translational research through application of specialized expertise in high-throughput genetic/genomic and protein analysis methods, together with related bioinformatics analysis. The core is located in the Research Center for Genetic Medicine at Children’s National Medical Center. Associated faculty have joint appointments in the Department of Integrative Systems Biology (ISB) at The George Washington University School of Medicine and Health Sciences.
The GPB core has state-of-the-art equipment purchased through institutional funds, philanthropic donations, and research grants, totaling over $10 million.
Whereas core facilities in most institutions are stand-alone units staffed by personnel dedicated to the core services, the GPB core typically aligns IDDRC projects with an interested ISB faculty member who shares research interests and/or technologies. This provides investigators with more guidance for their projects, from experimental design and data generation all the way to data analysis and interpretation.
In addition to the GPB core, ISB/Genetic Medicine also supports two other NIH cores: the National Center for Medical Rehabilitation Research Core and the Clinical and Translational Science Institute at Children’s National Medical Center. Together, these considerable resources offer substantial opportunities for collaboration, subject recruitment, and implementation of cutting-edge technology to address research questions. Many core functions are provided at highly subsidized charge-back that covers reagent costs.
A list of current GPB services available to IDDRC investigators includes:
- DNA extraction
- DNA banking
- TaqMan Allele Discrimination Assays (two units)
- BioTrove/ABI Highly Parallel TaqMan Assays
- Illumina Bead Arrays for CGH/CNVs
- GWAS-Affymetrix SNP chips or Illumina Bead Arrays
- DMET chips
- Next generation sequencing:
- RainDance Emulsion PCR (Highly Parallel Re-Sequencing)
- Pacific BioSciences Single Molecular Sequencing
- Illumina 24k site Epigenomics Panel (human only)
- Pacific BioSciences Epigenomics by Sequencing
- RNA/miRNA extraction and quality check
- Expression Profiling (mRNA):
- Affymetrix Genechips
- Illumina Bead Array
- Illumina RNASeq
- Expression Profiling (miRNA):
- Affymetrix Genechips
- Illumina Bead Array
- Illumina miRNASeq
- Life Technologies (Taqman RT-PCR cards)
- Taqman RT-PCR
- Western Blotting
- Flow Cytometry-Based Bead ELISA
High-Content Project Design and Data Analysis
- Protein identification from gel bands/spots or from solutions
- Proteome profiling or quantitative proteomics using both stable isotope labeling strategy (SILAC, 15N labeling) and label free strategy
- Consultation for specialized studies including assistance with experimental design, data analysis and interpretation
- Basic training in proteomics including sample preparation and use of bioinformatics tools for protein identification and quantification
- High-throughput/genomic statistics
- Data visualization
- Data dissemination-public repositories
- Project Design and Interpretation Support
- Custom software tools
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