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Cellular Imaging
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Retrovirally infected neural progenitors (green) migrated out of the SVZ after demyelination of the corpus callosum and were found ten days later in demyelinated lesions as mature myelinating oligodendrocytes [pNIT-GFP, green; GFAP, red; S100b, blue].(Published: Aguirre A, Dupree J, Mangin JM, and Gallo, V)
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Cellular imaging uses advanced computers, optics, and other equipment to allow researchers to observe the biological processes in individual cells. The Cellular Imaging Core has expanded operations on the new sixth floor of Children’s National Medical Center. Hosted by the Center for Neuroscience Research at Children’s Research Institute, this core facility provides cellular imaging services for all research studies and is directed by Tarik F. Haydar, PhD.
Services include
- Time-lapse multiphoton microscopy
- Confocal microscopy
- Calcium imaging
- Electrophysiology labs
- Production and maintenance of primary, organotypic slice, and cell line cultures
- Live cell videoimaging
- Three-dimensional reconstruction of brain cells based on microscopy images
- Ultrasound-guided embryonic surgery
- In utero electroporation
- Cell culture labs
A second facility at the George Washington University Center for Microscopy and Image Analysis offers additional equipment and support.
Equipment within this core facility includes:
- LSM 510 Meta NLO multiphoton/confocal microscope for time-lapse investigation in living brain tissue
- Stereo fluorescence Lumar microscope system
- ZEISS ApoTome grid microscope for optical sectioning and live imaging
- Multiple systems for calcium imaging, stereo dissecting, and patch clamping
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